《植物生理学报》 2011, 47(5): 475-480

大豆毛状根-VA菌根真菌双重培养体系的建立

李欣欣, 赵静, 廖红*

华南农业大学根系生物学研究中心, 广州510642

通信作者：廖红；E-mail: hliao@scau.edu.cn；Tel: 020-85283380

摘　要：

以大豆毛状根为宿主, 接种VA菌根真菌珠状巨孢囊霉(Gigaspora margarita), 经过3.5个月的双重培养, 观察到VA菌根 真菌珠状巨孢囊霉对大豆毛状根的侵染, 辅助细胞形成, 并获得VA菌根真菌成熟孢子, 在无菌条件下建立了大豆毛状 根-VA菌根真菌双重培养体系, 为研究菌根真菌侵染大豆根部形成共生体系及相关分子机制提供了一种有效的研究方法。

关键词：大豆毛状根; VA菌根真菌; 珠状巨孢囊霉; 双重培养

收稿：2011-01-03   修定：2011-03-31

资助：国家自然科学基金重大项目(30890132)

Establishment of Dual Culture System of Soybean [Glycine max (L.) Merr] Hairy Roots and Vesicular-Arbuscular (VA) Mycorrhizal Fungi

LI Xin-Xin, ZHAO Jing, LIAO Hong*

Root Biology Center, South China Agricultural University, Guangzhou 510642, China

Corresponding author: LIAO Hong; E-mail: hliao@scau.edu.cn; Tel: 020-85283380

Abstract:

This paper describes the processes of establishment of dual culture system of soybean hairy roots and vesicular-arbuscular (VA) mycorrhizal fungi Gigaspora margarita under axenic conditions. Soybean hairy roots were used as host to be infected by G. margarita. After 3.5 months of dual culture, the infection of transformed soybean hairy roots by VA mycorrhizal fungi was observed, subsequently auxiliary cells were formed and mature spores of G. margarita were obtained. The successful establishment of dual culture system of soybean and VA mycorrhizal fungi provides an effective method to study the mechanisms of symbiosis between soybean roots and VA mycorrhizal fungi and related molecular researches.

Key words: soybean hairy root; vesicular-arbuscular (VA) mycorrhizal fungi; Gigaspora margarita; dual culture system